Rapid targeted gene disruption in Bacillus anthracis
نویسندگان
چکیده
منابع مشابه
Rapid targeted gene disruption in Bacillus anthracis
BACKGROUND Anthrax is a zoonotic disease recognized to affect herbivores since Biblical times and has the widest range of susceptible host species of any known pathogen. The ease with which the bacterium can be weaponized and its recent deliberate use as an agent of terror, have highlighted the importance of gaining a deeper understanding and effective countermeasures for this important pathoge...
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An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.
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*Unité des Toxines et Pathogénie Bactérienne, Centre National de la Recherche Scientifique Unité de Recherche Associée 2172, Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris Cedex 15, France; and ‡Pharmacologie Moléculaire et Structurale, Institut National de la Santé et de la Recherche Médicale U266-Unité Mixte de Recherche 8600 Centre National de la Recherche Scientifique, Unité de Form...
متن کاملA rapid antimicrobial susceptibility test for Bacillus anthracis.
An effective public health response to a deliberate release of Bacillus anthracis will require a rapid distribution of antimicrobial agents for postexposure prophylaxis and treatment. However, conventional antimicrobial susceptibility testing for B. anthracis requires a 16- to 20-h incubation period. To reduce this time, we have combined a modified broth microdilution (BMD) susceptibility testi...
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In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these species to evaluate the potential of 16S rRNA gene sequencing as a diagnostic tool. We found eight distinct 16S types among all 107 16S rRNA gene seq...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2013
ISSN: 1472-6750
DOI: 10.1186/1472-6750-13-72